Lack of a chromosomal copy of the circular rDNA plasmid of entamoeba histolytica

Bagchi, Anindya ; Bhattacharya, Alok ; Bhattacharya, Sudha (1999) Lack of a chromosomal copy of the circular rDNA plasmid of entamoeba histolytica International Journal for Parasitology, 29 (11). pp. 1775-1783. ISSN 0020-7519

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00207...

Related URL: http://dx.doi.org/10.1016/S0020-7519(99)00125-3

Abstract

A number of small circular DNAs constitute a part of the genome of Entamoeba histolytica. Among them, the 24.5 kb circular DNA encoding rRNA (EhR1) is the most abundant. Pulsed field gel electrophoresis was used to determine if a chromosomal copy of EhR1 exists and what fraction of the total genome is circular. The results show that the chromosomes of E. histolytica are linear, and that no copy of EhR1 could be detected in any of the linear chromosomes.

Item Type:Article
Source:Copyright of this article belongs to Australian Society for Parasitology.
Keywords:Anomalous Migration; Circular DNA; Entamoeba histolytica; Exonuclease Ba/31; Extrachromosomal rDNA; Genome Mapping; Molecular Karyotype; Pulsed Field Gel Electrophoresis
ID Code:2730
Deposited On:08 Oct 2010 10:10
Last Modified:24 Jan 2011 05:40

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