Gupta, Richa ; China, Arnab ; Manjunatha, Ujjini H. ; Ponnanna, N. M. ; Nagaraja, Valakunja (2006) A complex of DNA gyrase and RNA polymerase fosters transcription in Mycobacterium smegmatis Biochemical and Biophysical Research Communications, 343 (4). pp. 1141-1145. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1016/j.bbrc.2006.02.195
Abstract
We report here the existence of a complex between RNA polymerase (RNAP) and DNA gyrase in Mycobacterium smegmatis. The interaction between the two enzymes was detected during our attempts to purify DNA gyrase from M. smegmatis. RNAP subunits co-eluted along with DNA gyrase in two different affinity chromatography column procedures employed to purify the latter enzyme. A complex containing both the enzymes was isolated through gel filtration chromatography and sucrose density gradient centrifugation of the cell free extracts. The complex exhibited both DNA supercoiling and transcription activities. Reduction in the transcription activity of the complex in the presence of DNA gyrase inhibitor indicates a role for DNA gyrase in stimulating transcription.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Affinity Chromatography; DNA Gyrase; Mass Spectrometry; Mycobacteria; RNA Polymerase |
| ID Code: | 27013 |
| Deposited On: | 08 Dec 2010 12:51 |
| Last Modified: | 25 Jan 2011 15:55 |
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