Hemavathy, Kirugaval C. ; Nagaraja, Valakunja (1995) DNA methylation in mycobacteria: absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences FEMS Immunology and Medical Microbiology, 11 (4). pp. 291-296. ISSN 0928-8244
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...
Related URL: http://dx.doi.org/10.1111/j.1574-695X.1995.tb00159.x
Abstract
The presence of 6-methyladenine and 5-methylcytosine at Dam (GATC) and Dcm (CCA/TGG) sites in DNA of mycobacterial species was investigated using isoschizomer restriction enzymes. In all species examined, Dam and Dcm recognition sequences were not methylated indicating the absence of these methyltransferases. On the other hand, high performance liquid chromatographic analysis of genomic DNA from Mycobacterium smegmatis and Mycobacterium tuberculosis showed significant levels of 6-methyladenine and 5-methylcytosine suggesting the presence of DNA methyltransferases other than Dam and Dcm. Occurrence of methylation was also established by a sensitive genetic assay.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Microbiological Societies. |
| Keywords: | Mycobacteria; DNA Methylation; dam; dcm |
| ID Code: | 26972 |
| Deposited On: | 08 Dec 2010 12:55 |
| Last Modified: | 31 Jan 2011 04:22 |
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