Isolation of sheep anterior pituitary messenger RNA and its translation in a heterologous cell-free system

Abstract

A preparation rich in the specific messenger RNA involved in the synthesis of prolactin from sheep anterior pituitary glands was obtained by employing both the immunochemical and affinity techniques. A dose-dependent and efficient stimulation of protein synthesis by the isolated total pituitary RNA as well as poly (A) rich RNA were achieved with the reticulocyte system. The synthesis of prolactin as one of the translational products of this cell-free system was established by specific immunoprecipitation followed by resolution on sodium dodecyl sulphate polyacrylamide gel electrophoresis.