A simplified method for the purification of riboflavin-binding protein from hen's egg yolk

Murthy, Usha S. ; Sreekrishna, K. ; Adiga, P. R. (1979) A simplified method for the purification of riboflavin-binding protein from hen's egg yolk Analytical Biochemistry, 92 (2). pp. 345-350. ISSN 0003-2697

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...

Related URL: http://dx.doi.org/10.1016/0003-2697(79)90668-7

Abstract

A simple and efficient procedure for the purification of the riboflavin-binding protein from hen's egg yolk is described. This method involves the removal by exclusion of lipoproteins and subsequent fractionation of soluble yolk proteins held on a DEAE-cellulose column by a salt gradient which is followed by purification by gel filtration on Sephadex G-100. The protein thus isolated is homogeneous by various physicoehemical, immunological, and functional criteria.

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