Characterization and physical map of choleraphage φ149 DNA

Sengupta, Anuradha ; Ray, Prabir ; Das, Jyotirmoy (1985) Characterization and physical map of choleraphage φ149 DNA Virology, 140 (2). pp. 217-229. ISSN 0042-6822

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/004268...

Related URL: http://dx.doi.org/10.1016/0042-6822(85)90360-5

Abstract

Choleraphage φ149 DNA is a linear double-stranded molecule 69 × 106 Da or 104 kilobase pairs (kbp). From restriction enzyme analysis, it has been concluded that the DNA is circularly permuted. There are at least three S1 nuclease-sensitive sites along the length of the molecule. These sites represent single-strand interruptions repairable by T4 DNA ligase. A physical map of the DNA has been constructed using the restriction endonucleases BamH1 and BglII.

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