Choudhury, Susanta Roy ; Bhadra, Rupak K. ; Das, Jyotirmoy (1994) Genome size and restriction fragment length polymorphism analysis of Vibrio cholerae strains belonging to different serovars and biotypes FEMS Microbiology Letters, 115 (2-3). pp. 329-334. ISSN 0378-1097
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/037810...
Abstract
The genome size of Vibrio cholerae has been determined by pulsed field gel electrophoresis following digestion of chromosomal DNA with endonucleases. The genome size of all the classical strains examined was about 3000 kb and that of El Tor biotype was 2500 kb. The NotI and SfiI digestion patterns of the genomes of several V. cholerae straimns belonging to different serovars and biotypes showed distinct restriction fragment length polymorphism (RFLP). RFLP analysis together with the genome size can be used to differentiate strains of different serovars and biotypes of V. cholerae
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Microbiological Societies. |
| Keywords: | Vibrio cholerae; Genome Size; Biotype; Serotype Differentiation; Pulsed Field Gel Electrophoresis; Restriction Fragment Length Polymorphism |
| ID Code: | 26540 |
| Deposited On: | 08 Dec 2010 13:35 |
| Last Modified: | 09 Feb 2011 07:51 |
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