Sarkar, Dhiman ; Bhaduri, Amar (1995) Temperature-induced rapid increase in cytoplasmic free Ca2+ in pathogenic Leishmania donovani promastigotes FEBS Letters, 375 (1-2). pp. 83-86. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...
Related URL: http://dx.doi.org/10.1016/0014-5793(95)01161-7
Abstract
We demonstrate that the cytoplasmic free Ca2+ concentration in three virulent Leishmania donovani promastigote strains is maintained at 20-30 nM level whereas the avirulent promastigotes maintain [Ca2+]i at 80-100 nM. Rapid exposure of virulent promastigotes to higher physiological temperature increases [Ca2+]i many-fold. Use of CaCl2, EGTA and Mn2+ confirmed that both release of Ca2+ from internal pool(s) and influx from outside milieu are responsible for this increase in [Ca2+]i. Application of oligomycin, CN-, and nigericin indicated the non-mitochondrial pHi sensitive pool as the possible intracellular origin for internal Ca2+ release. Exposure of these cells to acidic environment had no influence on [Ca2+]i. In contrast, the avirulent promastigotes or freshly isolated amastigotes had shown no effect of heat-shock or pH shock on [Ca2+]i. of these cells. Our results indicate that the upward shift in temperature may play a role in signal transduction events in morphogenetic transformation of L. donovani promastigotes that involves mobilization of Ca2+ in cytoplasm.
Item Type: | Article |
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Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
Keywords: | Heat Shock [Ca2+]i; Leishmania Donovani |
ID Code: | 26436 |
Deposited On: | 06 Dec 2010 12:32 |
Last Modified: | 17 May 2016 09:43 |
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