Sunnichan, V. G. ; Shivanna, K. R. ; Mohan Ram, H. Y. (1998) Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis Plant Cell Reports, 17 (12). pp. 951-956. ISSN 0721-7714
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Official URL: http://www.springerlink.com/content/7jrxc6u5dvh5ef...
Related URL: http://dx.doi.org/10.1007/s002990050516
Abstract
Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μm N6-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days. Shoots were rooted in vitro on 1/4-strength MS medium containing 9.82 μm indole-3-butyric acid. Nodulated callus was produced from hypocotyl explants cultured on MS medium supplemented with 4.52 μm 2,4-dichlorophenoxyacetic acid and 8.90 μm BAP. Somatic embryos developed when the nodulated callus was transferred to MS medium containing 0.45 μm thidiazuron (TDZ). TDZ treatment for 2 days gave the optimum response. Over 30% of the somatic embryos developed into plantlets when transferred to ¼-strength MS basal medium without any growth regulators. Plantlets produced from adventitious shoots and somatic embryos were acclimatized to ex vitro conditions and established in the field.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer-Verlag. |
Keywords: | Gum Karaya; Somatic Embryogenesis; Sterculia urens; Thidiazuron; Tree Tissue Culture |
ID Code: | 26421 |
Deposited On: | 06 Dec 2010 12:34 |
Last Modified: | 17 May 2016 09:43 |
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