Bhattacharyya, Anusree ; Bhattacharyya, Bhabatarak ; Roy, Siddhartha (1993) A study of colchicine tubulin complex by donor quenching of fluorescence energy transfer European Journal of Biochemistry, 216 (3). pp. 757-761. ISSN 0014-2956
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1432-...
Related URL: http://dx.doi.org/10.1111/j.1432-1033.1993.tb18195.x
Abstract
The utility of collisional quenching of energy donors in fluorescence energy transfer is described. In multi-donor single acceptor systems, which contain different classes of donors (as distinguished by their accessibility towards a collisional quencher), donor quenching may be used to assess the fraction of energy transfer from each class of donor. The tubulin-colchicine complex was used as a donor-acceptor system to show that two inaccessible tryptophans are at or near the colchicine binding site.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
ID Code: | 26256 |
Deposited On: | 06 Dec 2010 12:50 |
Last Modified: | 17 May 2016 09:34 |
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