Manual solid-phase syntheses of peptides on resins with high loading capacity requiring small volumes of solvents

Abstract

The synthesis of peptides by manual solid-phase methods requiring very small volume of solvents at different steps is described. The syntheses have been carried out on polystyrene-based resins using fluorenylmethoxy carboxyl chemistry. All reactions were carried out in vials, of capacity 1.2 or 6 ml, with gentle stirring. The volumes of solvents used were 1-2 ml for each operation. The crude peptides after cleavage from the resin were reasonably pure. The total volume of solvents required for the entire synthesis is substantially less as compared to volumes required in semi-automated and fully automated peptide synthesizers which renders the described method cost-effective.