Laxma Reddy, G. ; Nagaraj, R. (1986) Solid phase synthesis of the signal sequence of E. coli alkaline phosphatase Proceedings of the Indian Academy of Sciences - Chemical Sciences, 97 (1). pp. 71-75. ISSN 0253-4134
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Official URL: http://www.ias.ac.in/j_archive/chemsci/97/3/71-75/...
Related URL: http://dx.doi.org/10.1007/BF02880844
Abstract
A synthetic peptide corresponding to the signal sequence of E. coli alkaline phosphatase has been synthesized by the solid phase method employing the transesterification method of cleavage from the resin. The protected peptide obtained after cleavage was purified to homogeneity by column chromatography on silica gel, followed by partition chromatography on Sephadex LH-20 using organic solvents like chloroform and methanol as eluants.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Indian Academy of Sciences. |
| Keywords: | Signal Sequence; Solid Phase Synthesis; Transesterification; Partition Chromatography |
| ID Code: | 24198 |
| Deposited On: | 29 Nov 2010 10:13 |
| Last Modified: | 17 May 2016 07:56 |
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