Bramham Reddy, A. ; Gopinathan, K. P. (1986) Existence of single-strand interruptions in the genomic DNA of mycobacteriophage I3 FEMS Microbiology Letters, 37 (2). pp. 163-167. ISSN 0378-1097
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/037810...
Abstract
The genomic DNA of mycobacteriophage I3 harbours single-strand interruptions and is susceptible to degradation by nuclease S1, leading to random fragmentation. The native DNA serves as an efficient template for DNA polymerase I showing the presence of free 3'-OH termini which provide the priming points within the genome. The single-strand regions show no specificity for the 5'-end nucleotides flanking these interruptions and all 4 nucleotides are present with a probability equivalent to their percentage in the total DNA. The single-strand interruptions are an intrinsic character of this genome and are present irrespective of the host on which the phage is propagated.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Microbiological Societies. |
| Keywords: | Bacteriophage; Mycobacteriophage I3; DNA Polymerase I Genome; Single-strand Interruptions |
| ID Code: | 23669 |
| Deposited On: | 26 Nov 2010 08:50 |
| Last Modified: | 02 Jun 2011 06:16 |
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