Sadhu, Chanchal ; Dutta, Santanu ; Gopinathan, K. P. (1984) Influence of formamide on the thermal stability of DNA Journal of Biosciences, 6 (6). pp. 817-821. ISSN 0250-5991
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Official URL: http://www.ias.ac.in/jarch/jbiosci/6/817-821.pdf
Related URL: http://dx.doi.org/10.1007/BF02716841
Abstract
The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0.6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Indian Academy of Sciences. |
| Keywords: | DNA Melting; Formamide; Tm; DNA Base Composition; DNA Reassociation |
| ID Code: | 23653 |
| Deposited On: | 26 Nov 2010 08:47 |
| Last Modified: | 17 May 2016 07:26 |
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