Studies on malate or oxaloacetate formation from bicarbonate and pyruvate or phosphoenol pyruvate in cell-free extracts of Leishmania donovani

Abstract

1. 1. Complete organization of the cell components was essential for succinate production, since the cell-free extract of Leishmania donovani could not produce succinate from PEP or pyruvate by carboxylation reaction. But it could effectively produce oxaloacetate or malate from PEP or pyruvate and bicarbonate. 2. 2. Studies with a cell-free extract of the organism further showed that at least three different carboxylating systems were present in the preparation of the organism. 3. 3. The activities of NADP-dependent malic enzyme, NADH-dependent malate dehydrogenase and fumarase were shown to be present in the cell-free extract of L. donovani.