Effect of amines on fibrinogen synthesis

Abstract

L Epinephrine, serotonin, and isoproterenol stimulate the incorporation of [¹⁴C]leucine into thrombin-induced clottable protein; this stimulation was abolished by actinomycin D. The incorporation of ³²P into total RNA of rat liver, the site of fibrinogen synthesis, was stimulated by epinephrine and was highest at 2 h after ³²P administration. [¹⁴C]Orotic acid incorporation into polysomal RNA of liver was also increased significantly by epinephrine and serotonin. The immunoprecipitation of newly synthesized protein by monospecific antibody raised against pure rat fibrinogen clearly demonstrates that epinephrine increased fibrinogen formation in vivo under the experimental condition. Translation of poly (A)-containing RNA from total polysomal RNA clearly indicates that L-epinephrine increased mRNA specific for fibrinogen.