Madyastha, K. M. ; Ganguli, A. R. ; Kubair, V. G. ; Kowser, N. ; Vidya, D. (1987) Extracellular invertase from Aspergillus athecius: isolation and immobilization Biotechnology Letters, 9 (8). pp. 555-560. ISSN 0141-5492
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Official URL: http://www.springerlink.com/content/v6652j353k5883...
Related URL: http://dx.doi.org/10.1007/BF01026660
Abstract
A fungal strain isolated from soil and identified as Aspergillus athecius, when grown on moistened wheat bran produced large amounts of extracellular invertase. Most of the invertase from the moldy bran was easily extracted by low ionic strength buffer (0.005 M, pH 5.7). The crude invertase immobilized on DEAE cellulose showed not only increased activity (≈45%) but also greater thermal and storage stability than the free enzyme. The free and the bound enzymes showed a temperature optimum of 50-55°C and a pH optimum of 5.7 and 4.8 respectively. The Km app. of the bound enzyme was lower than that of the free enzyme.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer-Verlag. |
| ID Code: | 23489 |
| Deposited On: | 25 Nov 2010 13:14 |
| Last Modified: | 10 Jun 2011 06:38 |
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