Ahmad, Ateeq ; Bishayee, Subal ; Bachhawat, B. K. (1973) A novel method for immobilization of chicken brain Arylsulfatase A using Concanavalin A Biochemical and Biophysical Research Communications, 53 (3). pp. 730-736. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(73)90154-X
Abstract
Chicken brain Arylsulfatase A (E.C.3.1.6.1) was immobilized by interaction with Concanavalin A. The immobilized enzyme retained its catalytic activity and this enzyme can be reused without appreciable loss of activity. The storage stability of bound and soluble enzymes was comparable and binding of enzyme to Concanavalin A increases its thermal stability. Kinetic studies indicated that bound enzyme shows similar anomalous kinetics as that of free enzyme but slight change was observed in relation to pH optima, Km value and activation energy.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 2235 |
| Deposited On: | 07 Oct 2010 12:25 |
| Last Modified: | 14 May 2011 05:18 |
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