Generation of ROS in cells on exposure to CW and pulsed near-infrared laser tweezers

Mohanty, Samarendra Kumar ; Sharma, Mrinalini ; Gupta, Pradeep Kumar (2006) Generation of ROS in cells on exposure to CW and pulsed near-infrared laser tweezers Photochemical & Photobiological Sciences, 5 (1). pp. 134-139. ISSN 1474-905X

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Official URL: http://pubs.rsc.org/en/Content/ArticleLanding/2006...

Related URL: http://dx.doi.org/10.1039/b506061c

Abstract

We report the results of a study on generation of reactive oxygen species (ROS) and changes in the membrane potential of mitochondria of carcinoma of cervix (HeLa) and Chinese hamster ovary (CHO) cells following exposure to continuous wave (cw) or pulsed Nd: YAG laser (1064 nm). For a given laser irradiation, the generation of ROS and induced changes in the membrane potential of mitochondria were more pronounced for HeLa cells as compared to CHO cells. However, in both the cells the laser dose required to elicit a given change was much lower with pulsed laser exposure compared to that required with a cw laser exposure. This suggests involvement of photothermal effects in the laser irradiation induced changes. Mechanistic studies using quenchers for ROS suggest that laser irradiation leads to generation of hydroxyl radicals.

Item Type:Article
Source:Copyright of this article belongs to Royal Society of Chemistry.
ID Code:22329
Deposited On:23 Nov 2010 13:02
Last Modified:17 May 2016 06:23

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