Uppal, A. ; Kohli, R. ; Gupta, P. K. ; Dubey, P. S. (2003) Induction of phr gene expression in Escherichia coli strain KY706/pPL-1 by UVA (365 nm) irradiation Annals of Microbiology, 53 (3). pp. 329-336. ISSN 1590-4261
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Abstract
We report the induction of phr gene expression in the Escherichia coli strain KY706 pPL-1 by UVAirradiation. The induction of phr gene expression was monitored by the activity of β-galactosidase enzyme and was found to depend on the UVA dose and the post irradiation incubation period in the nutrient medium. Mechanistic studies carried out using quenchers and enhancer for singlet oxygen and hydroxyl radicals showed the involvement of these reactive oxygen species in the induced enzyme induction.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer-Verlag. |
| Keywords: | UVA Irradiation; SOS Repair; phr Gene Induction |
| ID Code: | 22325 |
| Deposited On: | 23 Nov 2010 13:03 |
| Last Modified: | 17 May 2016 06:23 |
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