Virulence of Pseudomonas aeruginosa cells surviving photodynamic treatment with toluidine blue

Sharma, Mrinalini ; Bansal, Harsha ; Gupta, Pradeep Kumar (2005) Virulence of Pseudomonas aeruginosa cells surviving photodynamic treatment with toluidine blue Current Microbiology, 50 (5). pp. 277-280. ISSN 0343-8651

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Official URL: http://www.springerlink.com/content/h3834u628t5586...

Related URL: http://dx.doi.org/10.1007/s00284-005-4473-1

Abstract

We report results of a study carried out to investigate photodynamic effect of Touildine blue (TBO) on virulent factors secreted by Pseudomonas aeruginosa. Viability of MCF-7 cells incubated with culture supernatants of bacteria photosensitized with TBO (experimental) was found to be higher than that for MCF-7 cells incubated with culture supernatants of bacterial cells treated either with TBO in dark (control II) or not receiving any treatment (neither TBO nor light (control I)). Furthermore, whereas MCF-7 cells incubated with supernatants of bacteria, control I and II, showed significant morphological alterations, no such changes were observed in MCF-7 cells incubated with supernatant of experimental cells. The activities of phospholipase C and proteases, virulent factors of P. aeruginosa, were also found to decrease on photosensitization. These results suggest that the potency of virulent factors is reduced in cells surviving photodynamic treatment with TBO and this may have implication in treatment of infections caused by P. aeruginosa.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
ID Code:22278
Deposited On:23 Nov 2010 08:41
Last Modified:16 Feb 2011 05:45

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