Appukuttan, P. S. ; Bachhawat, B. K. (1979) Separation of polypeptide chains of ricin and the interaction of the A chain with cibacron blue F3GA Biochimica et Biophysica Acta (BBA) - Protein Structure, 580 (1). pp. 10-14. ISSN 0005-2795
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...
Related URL: http://dx.doi.org/10.1016/0005-2795(79)90192-2
Abstract
From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose. The presence of a nucleotide-binding domain on the toxic chain A could be demonstrated from its interaction with the blue dye Cibacron Blue F3GA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Ricin Polypeptide Chain; Cibacron Blue F3GA; Galactomannan; Nucleotide-binding Domain |
| ID Code: | 2206 |
| Deposited On: | 08 Oct 2010 07:33 |
| Last Modified: | 12 May 2011 12:00 |
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