Trace amounts of enhancing factor/phospholipase A2 in mouse peritoneal exudate cells

Redkar, Abhay R. ; Kadam, Shilpa ; Deo, Madhav G. ; Mulherkar, Rita (1997) Trace amounts of enhancing factor/phospholipase A2 in mouse peritoneal exudate cells Journal of Biosciences, 22 (5). pp. 529-536. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/22/529-536.pdf

Related URL: http://dx.doi.org/10.1007/BF02703390

Abstract

Enhancing factor (EF), a mouse phospholipase A2 (PLA2), has been purified from the small intestines, based on its ability to increase the binding of epidermal growth factor in a radioreceptor assay. EF/PLA2 was found to be localized predominantly in the Paneth cells in the small intestines. Whether mouse intestinal EF/PLA2 is identical/similar to mouse secretory PLA2 was to be determined. Phospholipases are known to play a crucial role in the process of inflammation. This paper reports the presence of trace amounts of EF/PLA2 in the peritoneal exudate cells. Western blot analysis of the acid extracts showed the presence of a 14 kDa immunologically cross-reactive protein. RT-PCR analysis using EF specific primers amplified a ~700 bp product which was further confirmed to be EF-specific by nested PCR analysis and sequencing. Presence of EF in the peritoneal exudate cells could be a unique mode of transport of growth factor modulator to the site of injury to aid in regeneration/cell proliferation of damaged tissue.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Enhancing factor; Phospholipase A2; Inflammation; Neutrophil; Paneth cells; EGF
ID Code:21751
Deposited On:22 Nov 2010 10:52
Last Modified:17 May 2016 05:55

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