Histological effects of vitamin A on the tail-amputated tadpoles of Polypedates maculatus with special reference to homeotic transformation

Abstract

Vitamin A is a very common teratogen causing severe embryonic malformations and changes in pattern formation in amphibians. The effects of vitamin A include the induction of duplication of skeletal structures in the anteroposterior, proximodistal and dorsoventral axes. The purpose of this investigation was to study the histological changes in the regenerating tail of the tadpoles of Polypedates maculatus by treatment with vitamin A. The histological changes brought about by vitamin A included the development of multilayered epidermis and thickened basement membrane, enlargement of the notochord and the nerve cord and the clumping of muscles. In addition, single, paired and supernumerary homeotic limbs were visible from day 20 onwards at ectopic sites. Initially, the limb consisted of only procartilage cells. Muscles and digits appeared from day 48 onwards. In contrast, such observations were not found in the control tails. The significance of such changes is discussed.