Bhattacharya, Samir ; Das Gupta, Papiya ; Mukherjee, Sourabrata (1973) A comparative study of head and tail kidney peroxidase in a fish (Clarias batrachus) Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 44 (3). pp. 693-700. ISSN 0305-0491
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030504...
Related URL: http://dx.doi.org/10.1016/0305-0491(73)90218-6
Abstract
1. 1. A soluble supernatant (105,000 g) of Clarias batrachus head and tail kidney demonstrated high peroxidase activity. The specific activity of the head kidney enzyme was higher than that of the tail kidney enzyme. 2. 2. The pH optimum of the head kidney peroxidase was 4.0 whereas that of the tail kidney was 5.0. 3. 3. H2O2 at a concentration of 4.0 μmoles/ml was inhibitory to tail kidney peroxidase whereas head kidney peroxidase was inhibited at a concentration of 15.0 μmoles/ml. 4. 4. The roles of various peroxidase inhibitors and stimulators on the head and tail kidney peroxidases were studied and compared. 5. 5. Both head and tail kidney peroxidases were active in the peroxidation of iodide to triiodide (I3-) which was measured at 353 nm.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Peroxidase; Kidney Peroxidase; Clarias Batrachus |
| ID Code: | 21522 |
| Deposited On: | 22 Nov 2010 11:13 |
| Last Modified: | 20 Jan 2011 11:20 |
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