Lack of association between serum adiponectin levels and the Pro12Ala polymorphism in Asian Indians

Abstract

Aims: The aim of the study was to investigate the association of serum adiponectin levels with the Pro12Ala polymorphism of the peroxisome proliferator activated receptor- (PPARG) gene in Asian Indians. Methods: We selected 400 diabetic subjects, 200 with the Pro12Pro genotype (100 male and 100 female) and 200 with the Pro12Ala genotype (100 male and 100 female) and 400 age- and sex-matched normal glucose tolerance subjects with similar genotype profiles from the Chennai Urban Rural Epidemiology Study. Fasting serum adiponection levels were measured using radioimmunoassay. The Pro12Ala polymorphism was genotyped by PCR-restriction fragment length polymorphism using BstUI. Results: All clinical and biochemical parameters were similar in the subjects with the Pro12Pro and Pro12Ala genotypes. There was no significant difference in serum adiponectin values between subjects with the Pro12Pro and Pro12Ala genotypes (males 5.4 vs. 5.8 μg/ml, P = 0.546; females 6.9 vs. 7.2 μg/ml, P = 0.748). Adiponectin values did not differ among these two genotypes even when categorized based on their diabetes status (normal glucose tolerance Pro12Pro 7.9 vs. Pro12Ala 7.7 μg/ml, P = 0.994; diabetes Pro12Pro 4.7 vs. Pro12Ala 5.4 μg/ml, P = 0.622). Conclusion: The Pro12Ala polymorphism of the PPARG gene is not associated with serum adiponectin levels in Asian Indians.