Jagath-Reddy, J. ; Appaji Rao, N. ; Savithri, H. S. (1996) 3'non-templated 'A' addition by Taq DNA polymerase: an advantage in the construction of single and double mutants Current Science, 71 (9). pp. 710-712. ISSN 0011-3891
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Official URL: http://www.ias.ac.in/j_archive/currsci/71/9/710-71...
Abstract
The 3'-end non-templated 'A' addition by Tag DNA polymerase has been described as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3′ non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltransferase.
| Item Type: | Article |
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| Source: | Copyright of this article belongs to Current Science Association. |
| ID Code: | 21252 |
| Deposited On: | 20 Nov 2010 13:14 |
| Last Modified: | 17 May 2016 05:27 |
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