Nishikimi, Morimitsu ; Appaji Rao, N. ; Yagi, Kunio (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen Biochemical and Biophysical Research Communications, 46 (2). pp. 849-854. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1016/S0006-291X(72)80218-3
Abstract
The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition of superoxide dismutase. This observation indicated the involvement of superoxide aninon radical (O2-) in the reduction of NitroBT, the radical being generated in the reoxidation of reduced PMS. Similarly, the reduction of NitroBT coupled to D-amino acid oxidase-PMS system under aerobic conditions was also inhibited by superoxide dismutase. A simple method for detecting superoxide dismutase is described.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 21176 |
| Deposited On: | 20 Nov 2010 08:57 |
| Last Modified: | 12 May 2011 12:29 |
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