Singha, U. K. ; Bhakuni, Vinod ; Ali, Vahab ; Roy, Raja (1996) Leishmania donovani: metabolite mapping of promastigotes using proton nuclear magnetic resonance spectroscopy Molecular and Cellular Biochemistry, 162 (1). pp. 17-22. ISSN 0300-8177
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Official URL: http://www.springerlink.com/content/g984001550142n...
Related URL: http://dx.doi.org/10.1007/BF00250991
Abstract
Proton nuclear magnetic resonance spectroscopy was used for studying the intracellular metabolite profile of promastigotes of Leishmania donovani. The major intracellular metabolites observed in the promastigotes were acetate, alanine, succinate, glycine, α-glycerophosphorylcholine, acetoacetate, arginine and ethanol. A comparative study of the intracellular metabolite profile of promastigotes of different strains of L. donovani showed that, all the major intracellular metabolites were present in promastigotes of different strains. A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer-Verlag. |
| Keywords: | Leishmania donovani; Promastigotes; Metabolites; Nuclear Magnetic Resonance (NMR); Perchloric Acid Extract (PCA); Strain Differentiation |
| ID Code: | 21029 |
| Deposited On: | 20 Nov 2010 09:20 |
| Last Modified: | 17 Jan 2011 12:04 |
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