Physico-chemical characterization of a double long-chain cationic amphiphile (Vectamidine) by microelectrophoresis

Pector, Veronique ; Caspers, Jacques ; Banerjee, Sunanda ; Deriemaeker, Luc ; Fuks, Robert ; Ouahabi, Abdel. El. ; Vandenbranden, Michel ; Finsy, Robert ; Ruysschaert, Jean-Marie (1998) Physico-chemical characterization of a double long-chain cationic amphiphile (Vectamidine) by microelectrophoresis Biochimica et Biophysica Acta: Biomembranes, 1372 (2). pp. 339-346. ISSN 0005-2736

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00052...

Related URL: http://dx.doi.org/10.1016/S0005-2736(98)00074-1

Abstract

We recently synthesized a novel cationic amphiphile (N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine or Vectamidine (previously described as diC14-amidine)) that associates with DNA and RNA and facilitates their entry and expression into eukaryotic cells. Among several parameters that have been shown to influence the transfection process, the surface charge density plays a key role. Quantitative information about that charge density associated to the cationic amphiphiles organized in liposomal structure is not yet available. We provide here evidence by titration and microelectrophoresis measurements that an evaluation of the intrinsic acidity constants, the surface pH and the counterion binding constants allows to determine the charge density at physiological ρH of Vectamidine liposomes. The knowledge of this superficial charge is a prerequisite to a molecular understanding of the DNA-cationic amphiphile complex formation. The method described could be extended to any kind of cationic amphiphile.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Cationic Liposome; Microelectrophoresis; Zeta Potential
ID Code:20791
Deposited On:20 Nov 2010 13:33
Last Modified:12 Jan 2011 05:38

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