Bashamboo, A. ; Ali, S. (2001) Minisatellite associated sequence amplification (MASA) of the hypervariable repeat marker 33.15 reveals a male specific band in humans Molecular and Cellular Probes, 15 (2). pp. 89-92. ISSN 0890-8508
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S08908...
Related URL: http://dx.doi.org/10.1006/mcpr.2001.0350
Abstract
The 16-nt (5' CACCTCTCCACCTGCC 3') consensus sequence of the hypervariable repeat marker 33.15 was used as primer to conduct minisatellite associated sequence amplification (MASA) of human genomic DNA. MASA detected a 513-bp Y chromosome specific band in males as well as a number of variable bands ranging from 260-2700 bp in both the sexes. Overall polymorphism of the variable alleles was low with the band sharing probability of identity being 1.04×10-4. Cloning and sequence analysis of the male specific band (GenBank accession no. AF134482) showed a high level (60-80%) of sequence homology with several human Y chromosome derived clones. Similar analysis of Indian rhinoceros (Rhinoceros unicornis) genomic DNA showed male specific isomorphic band though of different size. This assay may provide a useful method for the detection of Y chromosome related heterochromatic sequences in human and other vertebrates.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Minisatellite Associated Sequence Amplification (MASA); Heterochromatic Sequences; Y Related Heterochromatin; |
| ID Code: | 20745 |
| Deposited On: | 20 Nov 2010 13:38 |
| Last Modified: | 11 May 2011 11:07 |
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