Chattopadhyaya, D. J. ; Nag, D. K. ; Mandal, N. C. (1983) Studies on polylysogens containing λN-cl- prophages II. Role of high multiplicities in lysogen formation by λN-cl- phage Virology, 128 (2). pp. 265-270. ISSN 0042-6822
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/004268...
Related URL: http://dx.doi.org/10.1016/0042-6822(83)90254-4
Abstract
Results of the experiments presented in this paper show that λN-cI- phage can lysogenize a nonpermissive host Escherichia coli when it infects at very high multiplicities (around 100), and λN-cI-cII- and λcIII-N-cI- lysogenize poorly at similar high multiplicities. The latter two phages lysogenize with appreciable frequency when either λN-cI- or λint-cN-cI-cII- is used as helper. The phages, λN-cI-, λN-cI-cII-, and λcIII-N-cI- can lysogenize also at relatively low m.o.i. of 20 in presence of the above λint-c helper, and the λint-cN-cI-cII- phage alone forms converted lysogens at an m.o.i. as low as 12. All these results suggest that the establishment of prophage integration by λN-cI- is positively regulated, like λN+cI+ phage, by the cII/cIII-promoted expression of the int gene of λ, and under the N- condition, high multiplicities are needed to provide optimum levels of cII and cIII products, especially the latter.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
ID Code: | 19953 |
Deposited On: | 20 Nov 2010 15:15 |
Last Modified: | 17 May 2016 04:24 |
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