Sengupta, T. K. ; Chaudhuri, K. ; Majumdar, S. ; Lohia, A. ; Chatterjee, A. N. ; Das, J. (1992) Interaction of Vibrio cholerae cells with beta-lactam antibiotics: emergence of resistant cells at a high frequency Antimicrobial Agents and Chemotherapy, 36 (4). pp. 788-795. ISSN 0066-4804
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Official URL: http://aac.asm.org/cgi/content/abstract/36/4/788
Abstract
Unlike other gram-negative enteric bacteria, Vibrio cholerae cells were equally susceptible to penicillin and ampicillin and in general more susceptible than Escherichia coli to most of the beta-lactam antibiotics. The turbidity of penicillin-treated cultures contained to increase exponentially for about 3 h, although the cell viability declined rapidly within 30 min of penicillin addition. Prolonged treatment with beta-lactam antibiotics produced cells resistant to these antibiotics. A fluctuation test indicated that this resistance might be due to adaptive mutation. Cells resistant to a beta-lactam exhibited broad cross-resistance to other beta-lactam antibiotics. A new 12,000-Da outer membrane protein was detected both in beta-lactam-resistant cells and in wild-type cells growing in medium containing beta-lactam antibiotics. While the penicillin-resistant cells had all of the penicillin-binding proteins (PBPs) present in the parental cells, significant differences in the relative proportion of low-molecular-weight PBPs were seen. The low-molecular-weight PBPs from resistant cells seemed to form more stable complexes with penicillin than those from the parental strain.
Item Type: | Article |
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Source: | Copyright of this article belongs to American Society for Microbiology. |
ID Code: | 19521 |
Deposited On: | 22 Nov 2010 12:27 |
Last Modified: | 17 May 2016 04:03 |
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