Characterisation of a repetitive DNA family from Entamoeba histolytica containing Saccharomyces cerevisiae ARS consensus sequences

Lohia, Anuradha ; Haider, N. ; Biswas, B. B. (1990) Characterisation of a repetitive DNA family from Entamoeba histolytica containing Saccharomyces cerevisiae ARS consensus sequences Gene, 96 (2). pp. 197-203. ISSN 0378-1119

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/037811...

Related URL: http://dx.doi.org/10.1016/0378-1119(90)90253-N

Abstract

Several repetitive DNA families were identified in Entamoeba histolytica DNA digested with Sau3AI. Characterisation of one of these repetitive DNA families showed the presence of multiple copies of Saccharomyces cerevisiae autonomously replicating sequence (ARS) core consensus sequences. The E. histolyticca ARS consensus sequences allowd a yeast-integrating plasmid, YIP5, to replicate autonomously in S. cerevisiae. A 'bent DNA' fragment was located in one member of this E. histolytica repetitive DNA family.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Protozoan Parasite; Recombinant Plasmid; Tandem Repeats; Sequence Analysis; Replication Origin; Bent DNA
ID Code:19479
Deposited On:22 Nov 2010 12:32
Last Modified:18 May 2011 11:27

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