Chattoraj, Sanghita ; Lahiri Majumder, Arun (1986) Modification of brain fructose-1, 6-bisphosphatase activity by chelators: "Induction" of 5'-AMP sensitivity Biochemical and Biophysical Research Communications, 139 (2). pp. 571-580. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1016/S0006-291X(86)80029-8
Abstract
Brain fructose-1, 6-bisphosphatase (EC 3.1.3.11) from various sources are ordinarily insensitive to 5'-AMP. In addition to stimulation and conferring a "neutral" behaviour, prior treatment with histidine, EDTA or imidazole renders the brain enzyme sensitive to 5'-AMP. The histidine treated enzyme(s) bind to Blue-Sepharose CL-6B column and are specifically eluted by 5'-AMP in contrast to the untreated enzyme(s) which do not bind to the affinity column at all. The histidine effect in inducing 5'-AMP sensitivity was abolished by treatment of the native enzyme by subtilisin or by a number of divalent cations including Zn++.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 19250 |
| Deposited On: | 23 Nov 2010 13:15 |
| Last Modified: | 06 Jun 2011 04:10 |
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