Pal, Santanu ; Ghosh, Shyamasree ; Bandyopadhyay, Suman ; Mandal, Chhabinath ; Bandhyopadhyay, Santu ; Bhattacharya, Dilip Kumar ; Mandal, Chitra (2004) Differential expression of 9-O-acetylated sialoglycoconjugates on leukemic blasts: a potential tool for long-term monitoring of children with acute lymphoblastic leukemia International Journal of Cancer, 111 (2). pp. 270-277. ISSN 0020-7136
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/ijc.202...
Related URL: http://dx.doi.org/10.1002/ijc.20246
Abstract
Earlier studies have demonstrated overexpression of 9-O-acetylated sialoglycoconjugates (9-O-AcSGs) on lymphoblasts, concomitant with high titers of anti-9-O-AcSG antibodies in childhood acute lymphoblastic leukemia (ALL). Our aim was to evaluate the correlation between expression of different 9-O-AcSGs during chemotherapeutic treatment. Accordingly, expression of 9-O-AcSGs on lymphoblasts of ALL patients (n = 70) were longitudinally monitored for 6 years (1997-2002), using Achatinin-H, a 9-O-acetylated sialic acid (9-O-AcSA) binding lectin with preferential affinity for 9-O-AcSGs with terminal 9-O-AcSAα2→6GalNAc. Western blot analysis of patients (n = 30) showed that 3 ALL-specific 9-O-AcSGs (90, 120 and 135 kDa) were induced at presentation; all these bands disappeared after treatment in patients (n = 22) who had disease-free survival. The 90 kDa band persisted in 8 patients who subsequently relapsed with reexpression of the 120 kDa band. FACS analysis revealed that at presentation (n = 70) 90.1 ± 5.0% cells expressed 9-O-AcSGs, which decreased progressively with chemotherapy, remained <5% during clinical remission and reappeared in relapse (80 ± 10%, n = 18). Early clearance of 9-O-AcSG+ cells, during 4-8 weeks of treatment showed a good correlation with low risk of relapse. Sensitivity of detection of 9-O-AcSG+ cells was 0.1%. Numbers of both high- and low-affinity binding sites were maximum at presentation, decreased with treatment and increased again in clinical relapse. We propose that close monitoring of 90 and 120 kDa 9-O-AcSGs may serve as a reliable index for long-term management of childhood ALL and merits therapeutic consideration.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
Keywords: | Achatinin-H; 9-O-acetylated Sialic Acid Binding Lectin; Acute Lymphoblastic Leukemia; Carbohydrate; Minimal Residual Disease; Sialic Acid |
ID Code: | 18982 |
Deposited On: | 25 Nov 2010 14:39 |
Last Modified: | 17 May 2016 03:37 |
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