Tyagi, Akhilesh K. ; Rashid, A. ; Maheshwari, S. C. (1981) Sodium chloride resistant cell line from haploid Datura innoxia mill. A resistance trait carried from cell to plantlet and vice versa in Vitro Protoplasma, 105 (3-4). pp. 327-332. ISSN 0033-183X
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Official URL: http://www.springerlink.com/content/x811802r07n754...
Related URL: http://dx.doi.org/10.1007/BF01279229
Abstract
A cell line resistant to sodium chloride was selected from callus cultures of haploid Datura innoxia by cloning under selective pressure. Cells of the resistant cell line retained their resistance even after subculture in absence of NaCl. Plantlets could be regenerated from resistant cells in the presence as well as absence of NaCl. In contrast, regeneration of plantlets was not possible from normal cells in the presence of NaCl, although regeneration readily occurred in the absence of NaCl. To examine the stability of the resistance in the long-term, callus cultures were initiated in presence of NaCl from stem expiants of the differentiated plantlets. All expiants of plantlets derived from resistant cells showed callus formation. This callus, derived from resistant explants, retained the trait of resistance upon subculture.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer-Verlag. |
Keywords: | Callus Culture; Datura innoxia; Plantlet Regeneration; Sodium Chloride Resistance |
ID Code: | 18657 |
Deposited On: | 17 Nov 2010 12:38 |
Last Modified: | 17 May 2016 03:21 |
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