Gill, Ravinder ; Rashid, A. ; Maheshwari, S. C. (1981) Dark requirement for cell regeneration and colony formation by mesophyll protoplasts of Nicotiana plumbaginifolia Viv Protoplasma, 106 (3-4). pp. 351-354. ISSN 0033-183X
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Official URL: http://www.springerlink.com/content/tg726074276104...
Related URL: http://dx.doi.org/10.1007/BF01275565
Abstract
The protoplasts of Nicotiana plumbaginifolia required darkness for cell regeneration and colony formation. Maximal plating efficiency of the protoplasts could be achieved by keeping the cultures in dark instead of light or dark/light sequence. Only two days of darkness prior to the illumination at 400 or 3,000 lux resulted in appreciable plating efficiency, than those of light from the beginning, but these values could not match the high plating efficiency in total darkness.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer-Verlag. |
Keywords: | Nicotiana; Dark Requirement; Protoplast Regeneration |
ID Code: | 18588 |
Deposited On: | 17 Nov 2010 12:45 |
Last Modified: | 17 May 2016 03:17 |
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