Androgenic response from cultured anthers of a leguminous tree, Cassia siamea Lam

Abstract

Anthers of Cassia siamea, on culture on B5 medium supplemented with coconut milk (15%, v/v), 2,4-D (2 mg/l), and kinetin (0.5 mg/l), split open after one to two weeks of inoculation and eject a callus mass. Microscopic examination of the anthers cultured at the late uninucleate or early bi-celled stages, after 7-14 days of culture, revealed many multicellular structures at various stages of development, thus indicating the pollen origin of callus. Callus cells also showed the haploid chromosome number (n=14).