Regeneration of plants from mesophyll protoplasts of Nicotiana plumbaginifolia Viv

Abstract

Protoplasts of Nicotiana plumbaginifolia were isolated by a one step enzymatic method. They were cultured in Ohyama and Nitsch's medium supplemented with 2,4-D (1.0 mg/l), benzylaminopurine (1.0 mg/l) and 14% sucrose. Cell divisions were initiated after 5 days and within 3 weeks colonies were discernible without the microscope. After transfer to Murashige and Skoog's medium containing IAA and kinetin the colonies differentiated into plantlets.