Enhancement of pollen embryo formation in Datura innoxia by charcoal

Tyagi, Akhilesh K. ; Rashid, A. ; Maheshwari, S. C. (1980) Enhancement of pollen embryo formation in Datura innoxia by charcoal Physiologia Plantarum, 49 (3). pp. 296-298. ISSN 0031-9317

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Official URL: http://www3.interscience.wiley.com/journal/1195871...

Related URL: http://dx.doi.org/10.1111/j.1399-3054.1980.tb02667.x

Abstract

In recent years liquid medium has been shown to be better than agar-gelled medium for production of haploids by anther culture. However, on addition of charcoal to agar medium the anther response in Datura innoxia Mill, increases dramatically and is better than in liquid medium. For anthers with pollen at the premitotic stage, the best result was observed with 1% charcoal in Difco agar and 1.5% in Normal agar. The effect is possibly due to adsorption of substances inhibitory to androgenesis and emanating from anthers, as well as to substances present in the nutrient medium and agar.

Item Type:Article
Source:Copyright of this article belongs to Scandinavian Society for Plant Physiology.
Keywords:Anther Culture; Charcoal; Datura innoxia; Pollen Embryo
ID Code:18515
Deposited On:17 Nov 2010 09:14
Last Modified:06 Jun 2011 11:51

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