Plant regeneration from protoplasts of Capsicum annuum L. cv. California wonder

Abstract

Plant regeneration from mesophyll protoplasts of pepper,Capsicum annuum L. cv. California Wonder has been demonstrated via shoot organogenesis. Protoplasts isolated from fully expanded leaves of 3-week-old axenic shoots when cultured in TM medium supplemented with 1 mg l ^-1 NAA, 1 mg l ^-1 2,4-D, 0 5 mg l ^-1 BAP (CM 1) resulted in divisions with a frequency ranging from 20-25 %. Antioxidant ascorbic acid and polyvinylpyrrolidone (PVP) in the medium and incubation in the dark helped overcome browning of protoplasts. Microcalli and macrocalli were formed in TM medium containing 2 mg l ^-1 NAA and 0.5 mg l ^-1 BAP (CM II) and MS gelled medium containing 2 mg 1 ^-1 NAA and 0 5 mg 1 ^-1 BAP (CM III), respectively. Regeneration of plantlets was possible via caulogenesis. Microshoots, 2-5 percallus appeared on MS gelled medium enriched with 0.5 mg l ^-1 IAA, 2mg l ^-1 GA and l0mg l ^-1 BAP (CM IVc). Rooting of microshoots was obtained on half strength gelled medium containing 1 mg l ^-1 NAA and 0.5mg l ^-1 BAP. Protoplasts isolated from cotyledons failed to divide and degenerated eventually.