Chidananda, Siddappa M. ; Satyamoorthy, Kapaettu ; Rai, Lavanya ; Manjunath, Attibele P. ; Kartha, Vasudevan B. (2006) Optical diagnosis of cervical cancer by fluorescence spectroscopy technique International Journal of Cancer, 119 (1). pp. 139-145. ISSN 0020-7136
![]()
|
PDF
- Publisher Version
568kB |
Official URL: http://onlinelibrary.wiley.com/doi/10.1002/ijc.218...
Related URL: http://dx.doi.org/10.1002/ijc.21825
Abstract
In the present work, we examine normal and malignant stage IIIB cervical tissue by laser induced fluorescence, with 2 different objectives. (i) Development of the fluorescence spectroscopy technique as a standard optical method for discrimination of normal and malignant tissue samples and, (ii) Optimization of the technique by the method of matching of a sample spectrum with calibration sets of spectra of pathologically certified samples. Laser-induced fluorescence spectra were measured using samples from 62 subjects at different excitation wavelengths. Principal component analysis (PCA) of spectra and intensity ratios of curve-resolved fluorescence peaks were tested for discrimination. It was found that PCA of total fluorescence at 325 nm excitation gives specificity and sensitivity over 95%. Use of calibration sets of spectra of histo-pathologically certified samples combined with PCA for matching and pass/fail classification of test samples is shown to have high sensitivity/specificity for routine diagnostic purposes as well as for possible staging of the disease. Further, the multi-component origin of the fluorescence spectra is illustrated by curve resolution and fluorescence spectra of separated proteins of tissue homogenates.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
Keywords: | Laser Induced Fluorescence; Optical Diagnosis; Principal Component Analysis |
ID Code: | 17900 |
Deposited On: | 16 Nov 2010 13:15 |
Last Modified: | 17 May 2016 02:35 |
Repository Staff Only: item control page