Bhat, Sujatha ; Patil, Ajeetkumar ; Rai, Lavanya ; Kartha, V. B. ; Santhosh, C. (2010) Protein profile analysis of cellular samples from the cervix for the objective diagnosis of cervical cancer using HPLC-LIF Journal of Chromatography: B, 878 (31). pp. 3225-3230. ISSN 1570-0232
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S15700...
Related URL: http://dx.doi.org/10.1016/j.jchromb.2010.09.025
Abstract
Protein profiles of cytologic samples from the cervix were studied using High Performance Liquid Chromatographic (HPLC) separation combined with ultra-sensitive laser induced fluorescence (LIF) detection. HPLC-LIF Protein profiles of samples from clinically normal subjects, individuals suffering from cervical cancer (different stages), and subjects who had other gynecological problems related to cervix, like erosion of cervix, Nabothian cyst etc., but no malignancy, were subjected to Principal Component Analysis (PCA). The application of HPLC-LIF protein profiling combined with PCA was found to be a highly efficient method for discrimination of different classes of samples with high sensitivity and specificity. Diagnostic accuracy and optimal threshold- decision criterion- for objective discrimination were estimated using sensitivity- specificity pairs and Youden's index (J) plots.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Cellular Samples; HPLC- LIF; Principal Component Analysis; Youden's Index |
| ID Code: | 17827 |
| Deposited On: | 16 Nov 2010 12:38 |
| Last Modified: | 03 Jun 2011 10:18 |
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