Pal, Moumita P. ; Vaidya, Bhalchandra K. ; Desai, Kiran M. ; Joshi, Renuka M. ; Nene, Sanjay N. ; Kulkarni, Bhaskar D. (2009) Media optimization for biosurfactant production by Rhodococcus erythropolis MTCC 2794: artificial intelligence versus a statistical approach Journal of Industrial Microbiology & Biotechnology, 36 (5). pp. 747-756. ISSN 1367-5435
![]()
|
PDF
- Publisher Version
413kB |
Official URL: http://www.springerlink.com/content/0k34428k21p235...
Related URL: http://dx.doi.org/10.1007/s10295-009-0547-6
Abstract
This paper entails a comprehensive study on production of a biosurfactant from Rhodococcus erythropolis MTCC 2794. Two optimization techniques-(1) artificial neural network (ANN) coupled with genetic algorithm (GA) and (2) response surface methodology (RSM)-were used for media optimization in order to enhance the biosurfactant yield by Rhodococcus erythropolis MTCC 2794. ANN and RSM models were developed, incorporating the quantity of four medium components (sucrose, yeast extract, meat peptone, and toluene) as independent input variables and biosurfactant yield [calculated in terms of percent emulsification index (% EI24)] as output variable. ANN-GA and RSM were compared for their predictive and generalization ability using a separate data set of 16 experiments, for which the average quadratic errors were ~3 and ~6%, respectively. ANN-GA was found to be more accurate and consistent in predicting optimized conditions and maximum yield than RSM. For the ANN-GA model, the values of correlation coefficient and average quadratic error were ~0.99 and ~3%, respectively. It was also shown that ANN-based models could be used accurately for sensitivity analysis. ANN-GA-optimized media gave about a 3.5-fold enhancement in biosurfactant yield.
Item Type: | Article |
---|---|
Source: | Copyright of this article belongs to Springer-Verlag. |
Keywords: | Biosurfactant; Media Optimization; Artificial Neural Network; Genetic Algorithm; Response Surface Methodology; Rhodococcus |
ID Code: | 17365 |
Deposited On: | 16 Nov 2010 08:20 |
Last Modified: | 17 May 2016 02:01 |
Repository Staff Only: item control page