Dandapat, P. ; Verma, Rishendra ; Venkatesan, K. ; Sharma, V. D. ; Singh, H. B. ; Das, R. ; Katoch, V. M. (1999) Rapid detection of Mycobacterium bovis on its lipid profile by thin layer chromatography Veterinary Microbiology, 65 (2). pp. 145-151. ISSN 0378-1135
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S03781...
Related URL: http://dx.doi.org/10.1016/S0378-1135(98)00279-X
Abstract
Sixteen Mycobacterium bovis (M. bovis) strains isolated from bovine tissues and one standard reference strain of M. bovis AN5 alongwith other species of mycobacteria for comparison were investigated for the presence of phenolic glycolipid (PGL) and phthiocerol dimycocerosate (PDIM) for rapid identification of M. bovis by thin-layer chromatography (TLC). The study indicated presence of PGL with an Rf value of 0.75 in chloroform-methanol solvent in all 17 M. bovis strains. The dimycocerostate A corresponding to spot A was the major constituent among all the three spots in M. bovis strains. TLC appeared to be a promising alternative to conventional biochemical methods for identification of M. bovis taking into consideration both PGL and PDIM lipids.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Mycobacterium Bovis; PGL; PDIM; Diagnosis-bacteria |
| ID Code: | 16851 |
| Deposited On: | 16 Nov 2010 13:27 |
| Last Modified: | 04 Jun 2011 10:02 |
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