Direct observation of (H8,H6)-Hr J-coupling correlations in oligonucleotides for unambiguous resonance assignments: use of J-scaling in two-dimensional correlated spectroscopy

Gundhi, Praful ; Chary, K. V. R. ; Hosur, R. V. (1985) Direct observation of (H8,H6)-Hr J-coupling correlations in oligonucleotides for unambiguous resonance assignments: use of J-scaling in two-dimensional correlated spectroscopy FEBS Letters, 191 (1). pp. 92-96. ISSN 0014-5793

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...

Related URL: http://dx.doi.org/10.1016/0014-5793(85)81000-0

Abstract

Four-bond H8/H6-H1 scalar coupling correlations in two-dimensional correlated spectroscopy have been observed directly for the first time by using the J-scaled COSY [(1985) Chem. Phys. Lett. 116, 105-108] technique in a dinucleotide, cytidylyl(2'-5')guanosine (CpG). Unambiguous resonance assignment of nonexchangeable protons in CpG has been obtained using these H8/H6-H1' 4-bond correlations and the various 3- and 4-bond sugar ring proton correlations observed in the COSY and SUPER COSY experiments.

Item Type:	Article
Source:	Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:	J-scaled COSY; 2D NMR; Oligonucleotide
ID Code:	16776
Deposited On:	15 Nov 2010 13:18
Last Modified:	17 May 2016 01:29

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