Application of two-dimensional NMR spectroscopy in the determination of solution conformation of nucleic acids

Abstract

Two-dimensional NMR has developed into an important tool for the study of solution structures of nucleic acids. The conformational information is generally derived from the three bond coupling constants (J) and interproton distances (r). Strategies have been developed to obtain J values using COSY and J-resolved spectroscopy. Estimation of interproton distances involves measurement of NOESY build-up curves as a function of mixing time. This information can be coupled with structural refinement techniques to obtain the three-dimensional structure of nucleic acids. Here we have described the current status of this important field with particular reference to advancements in applications to DNA structures.