Patel, P. K. ; Hosur, R. V. (1999) Application of 2D TOCSY to the determination of the stoichiometry of strand associations in multi-stranded DNA structures Magnetic Resonance in Chemistry, 37 (12). pp. 891-894. ISSN 0749-1581
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1...
Related URL: http://dx.doi.org/10.1002/(SICI)1097-458X(199912)
Abstract
A new strategy based on the use of the two-dimensional TOCSY experiment is described for the determination of the stoichiometry of association of strands in multi-stranded DNA structures. TOCSY spectra are recorded as a function of DNA concentration and the intensities of cross peaks are monitored to estimate the relative changes in the concentrations of the ordered and single-strand species at different total DNA concentrations. From these the stoichiometry and the association constant can be calculated. The method has been demonstrated on a quadruplex structure formed by the DNA segment d-AG3T.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
| Keywords: | Stoichiometry; 2D TOCSY; NMR; Multi-stranded DNA Structures |
| ID Code: | 16709 |
| Deposited On: | 15 Nov 2010 13:25 |
| Last Modified: | 03 Jun 2011 09:41 |
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