Patel, P. K. ; Bhavesh, Neel S. ; Hosur, R. V. (2000) NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG Biochemical and Biophysical Research Communications, 270 (3). pp. 967-971. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...
Related URL: http://dx.doi.org/10.1006/bbrc.2000.2479
Abstract
We report the NMR structure of the DNA sequence d-TGGGCGGT in Na+ solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH2-O2 H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10° compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | NMR Structure; G-quadruplex; C-tetrad; GGGCGG Repeat |
| ID Code: | 16705 |
| Deposited On: | 15 Nov 2010 13:25 |
| Last Modified: | 03 Jun 2011 09:42 |
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